P-126: Stem Cell Factor Increases Blastocyst Formation in Mouse Two-Cell Embryo Culture
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Abstract:
Background: It is demonstrated that c-Kit( receptor of stem cell factor) mRNA is expressed in late 2-cell stage to the expanded and hatched blastocyst and the stem cell factor (SCF) transcript is detected in the oviduct and uterus. The aim of this study was to investigate the effect of different doses of SCF on mouse 2-cell embryo development in vitro. Materials and Methods: 4-6 weeks old female mice were superovulated by i.p. injection of 7.5 IU PMSG and 46-48 hours later 7.5 IU hCG and caged overnight with males. 2-cell stage embryos were collected from oviducts and cultured in T6 medium supplemented with 0, 1, 20 and 50 ng/ml SCF to blastocyst stage then the number of blastocysts, hatching/ hatched and survived blastocysts was assessed. Results: The proportion of 2-cell embryos which developed to the blastocyst stage in group of 50 ng/ml SCF were significantly higher than control group (p<0.05). There was no difference in blastocyst formation between 1 and 20 ng/ml SCF and control groups (p>0.05). Hatching rate and blastocyst survival among control and treatment groups was not significant (p>0.05). Conclusion: Culture of mouse 2-cell embryos in the presence of SCF has beneficial effects on the rate of blastocyst formation.
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Journal title
volume 6 issue 2
pages -
publication date 2012-09-01
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